两种方法对比检测孕妇血型免疫性抗体

Comparison of gel microcolunm assay and conventional tube test for IgG antibodies against blood group antigens

目的分析比较凝胶微柱法与传统经典抗人球蛋白法测定O型孕妇血清中血型免疫性抗体效价的方法学差异。方法采用微柱凝集抗人球方法和经典抗人球蛋白试验平行检测77例与丈夫ABO血型不合的O型孕妇血清IgG抗A(B)水平,并对两种方法的结果进行方法学评价。结果分析测定77例样本,经统计学处理,两种方法之间的差异有统计学意义(P<0.01)。结论凝胶微柱法比试管间接抗人球法具有更高的灵敏度,且实验操作简便,快速,更适合于实验室内的广泛应用。

Objective To compare the gel microcolumn assay and the conventional tube test for IgG anti A （B） titration. Methods Seventy-seven blood samples of ＂O＂ group pregnant women, whose husbands were of non ＂O＂ group, were detected simultaneously by the conventional tube test and the gel microcolumn assay. The results were evaluated. Results Through the analysis of the results in 77 blood samples, IgG anti A （B） titration in the gel microcolumn assay showed significantly higher than that in the conventional tube test in all samples studied （P〈0.01）. Conclusion The gel microcolumn assay is superior to the conventional tube test. It has the further advantage of standardization of the test, shorter incubation time, and no washing procedure.