大鼠腰椎间盘针刺退变模型的建立

A rat model of lumbar intervertebral disc degeneration by an anulus needle puncture

[目的]建立大鼠腰椎间盘针刺退变模型并进行MRI和组织学观察。[方法]Sprague-Dawley大鼠32只,20只进行腰椎间盘纤维环厚度测量,确定针刺深度;12只经腹膜手术,L3、4、L4、5、L5、6椎间盘使用21G针分别行纤维环部分针刺或全层针刺,L6S1椎间盘作为对照,术前及术后4、8周行MRI检查,然后处死,椎间盘行HE染色。[结果]测量大鼠腰椎间盘纤维环厚度后,确定适用于L3、4、L4、5、L5、6、L6S1椎间盘纤维环部分针刺深度为1.5mm,纤维环全层针刺深度为2.3mm。MRI检查在纤维环全层针刺4周,部分针刺8周后的椎间盘信号强度显著降低。组织学检查发现纤维环全层针刺和部分针刺椎间盘4周时都发生退变,而纤维环全层针刺退变较严重。[结果]纤维环部分针刺或全层针刺方法可以成功建立大鼠腰椎间盘退变模型,方法可靠、有效、重复性好。

[ Objective] To establish a reproducible rat model of disc degeneration by puncturing the annulus with needles of defined gauges. [ Method ] Twenty skeletally mature male Sprague-Dawley rats were used to determine the depth of puncture by measuring anular thickness of lumbar disc. Twelve rats were carried out transperitoneal ventral surgery and anular stab was performed using a 21-gauge needle to make a full-thickness or partial-thickness stab incision in the L3,4, L4,5 and Ls,6 anterior- lateral disc. The L6Sl level was used as a control, Magnetic resonance images scans of the stabbed discs and control discs were performed at 4, 8-week time point and after MRI histological analysis were performed. [ Result] The depth of puncture was respectively partial-thickness 1.5 mm and full-thickness 2. 3 mm. The stabbed discs exhibited a decrease signal intensity from T2- weighted images starting at 4-week time point in full-thickness stab incision and 8-week in partial-thickness. Histological analysis revealed disc degeneration in both stab incision groups at 4-week time point and full-thickness stab incision displayed more severe degenerative changes compared with that of partial-thickness. [ Condusion] Full-thickness or partial-thickness anulus stab incision with needles of defined gauges is reliable, effective and reproducible to establish a rat model of intervertebral disc degeneration.