结核性脑膜炎动物模型的建立

Animal model of tuberculous meningitis

目的:建立结核性脑膜炎的小鼠模型。方法:C57BL系雄性小鼠眼球后静脉丛注射结核分枝杆菌H37RV0.35mg/0.05ml(1×106)建立结核性脑膜炎模型。4周后肉眼观小鼠脑的病变程度,并取脑组织进行匀浆培养,测定菌落数。结果:小鼠经结核分枝杆菌攻击后1周开始出现食欲降低,逐渐消瘦,被毛失去光泽,寒战,体温降低,活动量大幅度减少,攻击力逐渐下降至丧失;从感染后2周开始出现死亡,个别小鼠出现褪毛和行为异常;脑组织培养4周后,形成结核菌落。结论:此种方法成功建立了结核性脑膜炎动物模型。

Objective ：To establish the animal model of tuberculosis meningitis. Methods ： Mycobacterium tuberculosis H37 RV （ 0.35 mg/0.05 ml） was injected into C57 BL mice through the venous plexus behind the eyeball. After four weeks,the changes of brain tissue were observed, the brain tissue used for homogenate culture, and count of colony. Results ： After one week of Mycobacterium tuberculosis infection,the mice began to lose appetite and manifested athrepsy,tarnished clothing hair, chill and hypothemy; their activity amount decreased and aggressivity fell off to deprivation. After two weeks of infection,the mice began to die,some mice showed faded hair and abnormal behavior. Four weeks after the brain tissue was cultured, the tuberculosis colony was observed. Conclusions：It is feasible to establish the animal model of tuberculosis meningitis with this method.