枯萎病尖孢镰刀菌RAPD体系的建立

Establishment of RAPD Technique System on Fusarium Oxysporum

通过对PCR退火温度、反应体系中Mg2+、TaqDNA聚合酶、dNTPs、引物浓度等因子的优化,成功建立了枯萎病菌(Fusarium oxysporum)RAPD分析技术,并对其稳定性进行检测。结果表明在退火温度36℃,体系为TaqDNA聚合酶1.5 U,dNTPs浓度0.15 mM,Mg2+浓度1.5 mM,随机引物浓度30 ng时可以建立稳定灵敏度较高的RAPD分析体系。

RAPD technique optimization system for the pathogen Fusarium oxysporum DNA with clear bands was established by annealing tempreture in the amplification reaction, adjusting Mg^2＋ and Taq DNA polymerase, and optimiying the concentration of dNTPs and random primer. The stability of it was examined. The result showed the best satisfactory system was the concentration of random primer with 30 ng, Taq DNA polymerase 1.5 U, dNTPs 0.15 mM, MgCl2 1.5 mM and the annealing tempreature 36 ℃.