响应面法优化巴西虫草发酵培养基的研究

Optimization of Fermentation Medium for Cordyceps brasiliensis Using Response Surface Methodology

利用响应面法优化巴西虫草(Cordyceps brasiliensis)液态发酵培养基。在预备实验的基础上,采用Plankett-Burman设计对影响巴西虫草菌丝体产量的液态发酵培养基组分进行了筛选,确定主要影响因子为葡萄糖和牛肉膏。再用最陡爬坡实验逼近以上2个因素的最大响应区域。最后通过中心组合设计和响应面分析法,确定了主要影响因子的浓度。优化后的培养基组成为:葡萄糖4.46g/100mL,可溶性淀粉2g/100mL,蚕蛹粉4g/100mL,牛肉膏2.93g/100mL,KH2PO40.1g/100mL和MgSO4·7H2O0.15g/100mL。

The composition of fermentation medium for producing Cordyceps brasiliensis mycelium was optimized using response surface methodology. A Plackett-Burman design was applied to screen potential key factors selected on the basis of preliminary experiments, and the steepest ascent method was adopted to approach the approximate optimal medium composition. Finally, central composite design and response surface methodology were applied to define more precisely the optimal composition of the fermentation medium. Our data revealed glucose and beef extract to be the most influential parameters, and the composition of the optimal fermentation medium （100 mL） was determined to consist of 4.46 g glucose, 2 g soluble starch, 4 g silkworm powder, 2.93 g beef extract, 0.10 g KH2PO4 and 0.15 g MgSO4·7H2O.