脾胃湿热证与人类白细胞抗原Ⅱ类基因多态性的相关性研究

Correlation of Spleen-stomach Damp-heat Syndrome with Human Leucocyte Antigen Class Ⅱ Gene Polymorphism

【目的】探讨广东地区汉族人群慢性胃炎、消化性溃疡患者中脾胃湿热证与人类白细胞抗原(HLA)-Ⅱ类等位基因的相关关系。【方法】选择以籍贯为广东地区的汉族健康人(正常对照组16例)和慢性浅表性胃炎或消化性溃疡患者(观察组46例,根据辨证分为脾胃湿热证组26例、脾气虚证组20例)的全血为研究标本,采用聚合酶链反应—序列特异性引物(PCR-SSP)基因分型方法检测HLA-Ⅱ类基因中的HLA-DQB1、HLA-DRB1、HLA-DQA1及HLA-DPB1的等位基因类型。【结果】DQA1*0103基因型在脾胃湿热组显著高于正常对照组(P<0.05),但与脾气虚组比较无显著性差异;DQA1*0505基因型在脾胃湿热组显著低于正常对照组及脾气虚组(P<0.05)。【结论】脾胃湿热证存在着一定的免疫遗传基础,DQA1*0103基因型可能是脾胃湿热证的易感基因,DQA1*0505可能是脾胃湿热证的保护性基因。

[Objective] To study the correlation of human leucocyte antigen （HLA） class Ⅱ allele with spleen-stomach damp-heat （SSDH） syndrome. [Methods] From those people with native place of Guangdong, 16 healthy volunteers served as the normal control and 46 patients with chronic superficial gastritis or digestive ulceration were enrolled into the observation. Of 46 patients, 26 were classified into SSDH and 2Ointo spleen deficiency （SD） syndrome. HLA-DRB1, HLA-DQA1, HLA-DQB1, and HLA-DPB1 alleles of HLA class Ⅱ from whole blood sample were analyzed by the method of polymerase chain reaction-sequence specific primers （PCR-SSP）. [Results] The gene frequency of DQA1^＊ 0103 in SSDH group was remarkably higher than that in the normal control group （ P 〈 0.05 ）, but no statistical significant difference was found between SSDH group and SD group. Gene frequency of DQA1^＊ 0505 in SSDH group was remarkably lower than the normal and SD groups （P 〈 0.05）. [Conclusion] There exists possible immunogenetic basis for SSDH syndrome, and DQA1 ^＊ 0103 may be the susceptible gene and DQA1 ^＊ 0505 the protective gene of SSDH syndrome.