补中益气丸对脾虚大鼠甲状腺激素水平的影响

Effect of Buzhong Yiqi Wan on Thyroid Hormones in Rat Model of Spleen-asthenia

【目的】观察补中益气丸对实验性脾虚证大鼠甲状腺激素水平的影响。【方法】选SD雄性大鼠34只,随机分为3组:即正常对照组(N=10)、脾虚模型组(N=12)、补中益气丸组(N=12)。除正常对照组外,其他组均采用大黄(4 g.d-1.只-1)水煎液复制脾虚证动物模型,补中益气丸组同时灌服补中益气丸(剂量为3.9 g.kg-1.d-1),均连续20 d。采用放射免疫法检测各组动物血清三碘甲腺原氨酸(T3)、甲状腺素(T4)、3,3,5—反三磺甲腺原氨酸(rT3)、促甲状腺素(TSH)水平的变化,并观察各组动物体质量,脾脏、胸腺质量及其与体质量的比值。【结果】脾虚模型组大鼠血清T3、T4含量,脾脏、胸腺质量及其比值均较正常对照组显著性下降(P<0.01),而补中益气丸能提高实验性脾虚证大鼠T3、T4水平及胸腺组织质量与其比值(P<0.05或P<0.01)。【结论】补中益气丸治疗脾虚的作用机理可能与调节甲状腺激素水平有关。

[ Objective] To observe the effect of Buzhong Yiqi Wan （BYW） on thyroid hormones in rat model of spleenasthenia. [Methods] Thirty-four SD male rats were randomized into 3 groups： normal control group （ N = 10）, spleenasthenia model group （ N = 12）, BYW group （ N = 12）. Except the normal control group, the rats in other groups were given the decoction of Radix et Rhizoma Rhei （ 4 g·d^- 1 for each rat） to induce spleen asthenia, and meanwhile, BYW group reveived BYW （3.9 g·kg^-1·d^-1）. The treatment lasted 20 days. Radioimmunoassay was used to detect serum levels of triiodothyronine （T3）, thyroxine （T4）, reverse triiodothyronine （rT3） and thyroid stimulating hormone （TSH）. Meanwhile, the body weight, spleen and thymus weight as well as their ratio with the body weight were also observed. [Results] Serum T3 and 374 levels, spleen and thymus weight as well as their ratio with the body weight were decreased in the model group as compared with those in the normal control group （P 〈 0.01）, and BYW increased the T3 and T4 levels as well as the ratio of thymus weight with body weight （ P 〈 0.05 or P 〈 0.01）. [Conclusion] The therapeutic effect of BYW for spleen asthenia may be related to the regulation of thyroid hormones levels.