FLIP、NF-κB在胃癌中的表达及相关性研究

A Study of Relationship between Nuclear Factor KappaB(NF-κB) and FLICE-inhibitory Protein(FLIP) Expression and Gastric Carcinoma

[目的]研究FLIP、NF-κB在胃癌及癌前病变中的表达及两者之间的关系。[方法]运用免疫组织化学染色SP法检测FLIP、NF-κB在45例胃癌与60例癌前病变组织中的表达。[结果]胃癌FLIP、NF-κB蛋白阳性表达分别为82.2%(37/45)、62.2%(28/45),明显高于肠化生的30.0%(9/30)、23.3%(7/30)(P<0.01),与异型增生40.0%(12/30)、33.3%(10/30)(P<0.01)。FLIP表达与胃癌临床分期及淋巴结转移相关,与肿瘤分化程度无明显相关性;NF-κB表达与胃癌分化程度、临床分期及淋巴结转移相关(P<0.05)。NF-κB与FLIP呈正相关(r=0.52,P<0.05)。[结论]FLIP与NF-κB异常表达与胃癌的发生发展及预后密切相关;NF-κB与FLIP在胃癌形成中可能起协同作用。

[Purpose] To explore the relationship of FLICE （FADD （Fas-associated death domain）-like IL-1 beta-converting enzyme ）-inhibitory protein （FLIP） and NF-κB expression and gastric carcinoma and precancerous lesions. [Methods] Expression of FLIP and NF-κB in 45 cases with gastric carcinoma and 60 cases with precancerous lesion was detected by innnunohistoehemical method （SP method）. [Results] Positive expression of FLIP and NF-KB in gastric carcinoma was 82.2%（37/45） and 62.2%（28/45）; in intestinal metaplasia, 30.0%（9/30） and 23.3%（7/30）; and in dysplasia, 40.0%（12/30） and 33.3%（10/30） respectively with significant difference （P〈0.01）. Expression of FLIP correlated with clinical staging and lymph node metastasis, not with differentiation degree in gastric c, arcinoma, Expression of NF-κB correlated with differentiation degree, clinical staging and lymph node metastasis in gastric carcinoma（P〈0.05）. There was a positive correlation between FLIP and NF-κB （r=0.52,P〈0.05）. [Conclusion] Abnormal expression of FLIP and NF-κB closely correlates with gastric carcinogenesis and prognosis. NF-κB and FLIP may play a synergistic role in gastric carcinogenesis.