土壤脲酶抑制剂正丁基硫代磷酰三胺的作用基团研究

Functional Groups of Soil Urease Inhibitor nBPT

土壤脲酶抑制剂正丁基硫代磷酰三胺(nBPT)是抑制土壤中尿素水解的最有效的化合物之一。分析大连工业大学合成的土壤脲酶抑制剂nBPT抑制脲酶活性的影响因素及作用机理,结果表明:在50°C,pH=5.91时,nBPT的抑制活性达到最大值;在nBPT各结构基团中,正丁基(-NH(CH2)3CH3)、硫基(-S)对nBPT与脲酶的结合起辅助作用,胺基(-NH2)是nBPT与脲酶结合的关键基团,与脲酶活性部位巯基(-SH)结合。土壤脲酶抑制剂nBPT与脲酶的具体结合机理还有待继续研究。

N-（n-butyl） tiophosphoric triamide （nBPT） is one of the most efficient soil urease inhibitors that suppress urea hydrolysis in the fields, thus preventing ammonia loss, and increasing N use efficiency of urea. This paper was to explore functional groups ofnBPT, their mechanisms and influencing factors, for example, temperature and pH, and a method to purify the urease from soybean. The result showed that 50 ℃ was the optimum temperature, 5.91 the optimum pH, and -NH2 the key functional group.