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cDNA末端快速扩增技术及其在血液疾病上的应用

Rapid Amplification of cDNA Ends and Its Application in Hematological Disease
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摘要 克隆新基因及获得其cDNA全长是现代生命科学中研究基因功能的重要前提。以PCR为基础的cDNA末端快速扩增(RACE)技术是一种简单、敏感且有效扩增cDNA全长的方法。该文主要概述RACE技术的原理、进展及其在血液疾病基因克隆方面的应用。 Cloning a novel gene and obtaining its full-length cDNA are crucial prerequisite for the functional annotation of genes. RACE （ Rapid Amplification of cDNA Ends） technique based on PCR is one simple method used to generate full-length cDNA efficiently and rapidly. In the article, the principle and improvement of the techniques were analyzed,and the application of the techniques in hematological disease were also reviewed in detail.
作者 王程毅 王少元
机构地区 福建医科大学附属协和医院血液科福建省血液病研究所
出处 《医学综述》 2007年第1期8-10,共3页 Medical Recapitulate
基金 福建省重大科研项目资助课题(2003F003)
关键词 RACE 全长CDNA 应用 RACE Full-length cDNA Application
分类号 R733.7 [医药卫生—肿瘤]
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参考文献13

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