海南红厚壳花浸膏、精油的提取及化学成分分析

Extraction and Analysis of the Essential Oil from the Flowers of Calophyllum inophyllum Linn

分别用水蒸气蒸馏法、溶剂法以及CO2超临界萃取法,对海南红厚壳鲜花进行精油、浸膏提取。3种不同提取方法的得油率分别为0.059%、0.33%、0.20%。用GC-MS技术对其进行了化学成分分析鉴定,并比较不同提取方法所得产物化学成分的异同。水蒸气蒸馏法所得精油共鉴定出25种化合物,主要成分为w(大根香叶烯)=22.62%、w(反式-石竹烯)=12.03%、w(β-倍半水芹烯)=19.00%;石油醚浸提所得浸膏鉴定出25种化合物,主要成分为w(大根香叶烯)=21.24%、w(β-倍半水芹烯)=9.07%、w(高香叶烯)=5.69%;超临界CO2萃取所得浸膏鉴定出19种化合物,主要成分为w(大根香叶烯-D)=17.70%、w(反式-石竹烯)=15.43%、w(16-贝壳松烯)=11.55%、w(α-柯拜烯)=10.62%。

The concrete and essential oil from the fresh flower of Calophyllum inophyllum Linn were obtained by steam distillation ,petrol-ether extraction and SCF extraction in yields of 0. 059% ,0. 33% and 0. 20% respectively. The chemical components of the oil were examined using the GC - MS technique ,and the differences of the major constituents of the oil acquired by three methods were compared. 25 compounds in the oil by steam distillation were identified, the principal constituents being germacrene-D （ 22.62% ）, trans-caryophyllene （ 12.03% ） and/3-sesquiphellandrene （ 19.00% ）. 25 compounds in the oil produced by petrol-ether extraction were identified, the principal constituents being germacrene-D （ 21.24% ）,β-sesquiphellandrene （ 9.07% ） and dodecatriene （ 5.69% ）. 19 compounds in the oil by SCF extraction were identified, the principal constituents being germacrene-D （ 17. 7% ） ,trans-caryophyllene（ 15.43% ）,kaur-16-ene（ 11.55% ） and α-copaene（ 10. 62% ）.