mDRA-6与尼美舒利对Jurkat细胞的杀伤效应

Lethal effect of mDRA-6 and nimesulide on Jurkat cells

目的:探讨mDRA-6与尼美舒利对Jurkat细胞有无杀伤作用及二者有无协同效应。方法:常规培养Jurkat细胞,流式细胞术检测细胞表面DR5的表达率,MTT法检测细胞毒性作用,Hoechst33258染色观察Jurkat细胞核形态变化,流式细胞术定量分析凋亡细胞率。结果:①Jurkat细胞表面DR5的表达率为84.83%。②mDRA-6与尼美舒利均能杀伤Jurkat细胞,存在浓度依赖性。400μmol/L的尼美舒利作用细胞10小时,细胞凋亡率为11.51%;0.5ng/ml与1ng/ml的mDRA-6作用细胞10小时,细胞凋亡率分别为3.67%和6.3%;③二者联合具有较好的协同促凋亡作用,400μmol/L的尼美舒利联合0.5ng/ml与1ng/ml的mDRA-6作用细胞10小时,细胞凋亡率分别为50.38%和63.79%。结论:mDRA-6与尼美舒利均有杀伤Jurkat细胞的作用,二者具有较强的协同作用,杀伤作用是通过诱导凋亡实现的。

Objective：To study the lethal effect of either anti-human DR5 monoclonal antibody（mDRA-6） or nimesulide, and their synergistic cytotoxicity against Jurkat cells and the possible mechanism. Methods ：Jurkat cells were cultured with RPMII640 meditum in regular condition. The morphology was observed under microscope. Cytotoxicity was examined by MTT assay. Apoptosis was detected by flow cytometry. Results：（1）Expression rate of DR5 on Jurkat cells was 84. 83%. （2）Both mDRA-6 and nimesulide were able to kill Jurkat cells, respectively. Concentration：dependent cytotoxicity of mDRA-6 and nimesulide was exhibited. Treated with 400 μmol/ L nimesulide for 10 h, 11.51% Jurkat cells were apoptotic. Treated with either 0. 5 ng/ml or 1 ng/ml of mDRA-6 for 10 h, 3.67% or 6. 3% of Jurkat cells were apoptotic, respectively. （3）The combination of mDRA-6 and nimesulide exhibited synergistic effect on Jurkat cells. 400 μmol/L nimesulide combined with 0. 5 ng/ml or 1 ng/ml mDRA-6 could make 50. 38% and 63.79% Jurkat cells apoptotic, respectively. Conclusion： Both mDRA-6 and nimesulide can induce Jurkat cell apoptosis. The combination of mDRA-6 and nimesulide exhibit synergistic cytotoxicity to Jurkat cells.