标准菌株和临床菌株oipA基因的检测及其核苷酸序列比对

Detecting of gene oipA of normal and clinical Helicobacter pylor strains and comparing of their nucleotide sequences

目的:检测幽门螺杆菌标准菌株NCTC11637及临床分离菌株Hp1和Hp2的oipA基因,分析其核苷酸序列,比对其与国际标准菌株Hp26695的同源性。方法:常规方法培养幽门螺杆菌,提取DNA,PCR法扩增oipA基因,检测其核苷酸序列,并比较其与Hp26695的同源性。结果:NCTC11637及Hp1、Hp2均表达oipA基因。其核苷酸序列与Hp26695比对,NCTC11637有48个突变位点、Hp1有48个突变位点、Hp2有50个突变位点,同源性均为94%。NCTC11637与Hp1的同源性为100%、与Hp2的同源性为97%。结论:NCTC11637、Hp1、Hp2均表达oipA基因,但不同菌株oipA基因的核苷酸序列有所不同。

Objective：To detect the oipA gene of Helicobacterpylori（Hp） strains NCTC11637 and Hp1, Hp2 isolated from clinical biopsies, analyze their nucleotide sequences and make a homologous comparison of nucleotide with Hp 26695. Methods：The oipA gene was detected with PCR in Helicobacter pylori （Hp） strains NCTC11637 and Hp1, Hp2 isolated from clinical gastric biopsies after routine culture. Then PCR products were sent out for nucleotide sequence analysis and compared with Hp 26695. Results：The sequence of the aim gene was obtained in NCTC11637 and Hp1, Hp2 and was made a homologous comparison of nucleotide with 26695. The number of mutation of NCTC11637, Hp1 and Hp2 and was 48,48,50 respectively. The identity was 94%, 94% and 94% respectively, while the strain Hp1 was most identical to 11637 as much as 100%. The homology of lip2 and 11637 was 97%. Conclusion：Hp1, Hp2 and NCTC11637 expresse gene oipA, but the sequences of gene oipA of different strains are distinct.