湖南地区白血病MHC-Ⅰ类链相关基因A微卫星多态性分析

Association of MICA-STR with leukemia in Hunan Han population

目的:探讨MHC-Ⅰ类相关链基因A(MHC class-Ⅰchain related gene A,MICA)与湖南地区白血病之间的相关性。方法:应用荧光聚合酶链反应-基因扫描技术和聚合酶链反应-序列特异性引物技术分析,对62例白血病患者和112名正常人群进行MICA基因第5外显子微卫星等位基因分型及MICA基因缺失检测。结果:慢性粒细胞白血病组(n=35)的MICA*A5基因频率显著低于正常对照组(RR=0.635,P=0.0380);急性淋巴细胞性白血病组(n=13)的MICA*A4基因频率显著低于正常对照组(RR=0.120,P=0.0297);而在急性非淋巴细胞性白血病组(n=14),MICA*A5基因频率显著高于正常对照组(RR=2.229,P=0.0218)。结论:本文数据显示,MICA-STR多态性与湖南地区白血病之间存在相关性;不同病理类型的白血病相关格局有所不同。

Objective：To explore the association between the short tandem repeat polymorphism of exon 5 of MICA gene （MICA-STR） and leukemia in Hunan Han population. Methods：62 patients with leukemia and 112 randomly selected normal controls residing in Hunan province were analyzed for MICA-STR allelic variation and MICA gene deletion by fluorescent pelymerase chain reaction- genescanning and pelymerase chain reaction-sequence specific priming. Results：The overall comparison showed no significant difference of MICA-STR frequency between patient group and control group （ P 〉 0, 05 ）. Subsequent stratification revealed that MICA ＊ A5 was present at significantly lower frequency in patient group with chronic granulocytic leukemia（CML） （RR =0. 635, P =0. 038 0） ; MICA ＊ A4 at significantly lower frequency in patient group with lyphololastic leukemia（ALL） （ RR = 0. 120, P = 0. 029 7 ） ; MICA ＊ A5 at significantly higher frequency in patient group with acute non-lyphololastic leukemia（ANLL） （ RR =2. 229, P =0. 021 8） ,all compared with control group. Conclusion：MICA-STR allelic variation is associated with leukemia in Hunan Han population; The data also suggest the heterogeneity of MICA-STR allele associated with different clinical types of leukemia.