CD64在早产儿细菌性感染中的应用价值

The evaluation of CD64 detection in diagnosing premature bacterial infection

目的:探讨中性粒细胞表面标志物CD64在早产儿感染中的诊断价值。方法:对新生儿科(包括NICU)109例患儿于流式细胞仪进行CD64水平的测定,并与C反应蛋白(CRP)(免疫比浊法检测)及IL-6(双抗体夹心ELISA法检测)进行比较,分早产儿感染组30例、足月儿感染组28例、早产儿非感染组25例和足月儿非感染组26例4组进行分析。结果:早产儿感染组CD64为(5010.95±3318.01)分子数/细胞;足月儿感染组CD64为(5193.07±3207.32)分子数/细胞;早产儿非感染组CD64为(2277.80±914.56)分子数/细胞;足月儿非感染组为(2174.34±836.00)。早产儿感染组与早产儿非感染组、足月儿感染组与足月儿非感染组比较CD64水平差异非常显著(P<0.001),早产儿感染组与足月儿感染组、早产儿非感染组与足月儿非感染组CD64水平无统计学差异;CD64诊断早产儿感染的敏感度、特异度均较高(敏感度76.7%、特异度88%),优于CRP(敏感度56.7%、特异度62%)及IL-6(敏感度64.3%、特异度84%)。结论:新生儿感染时CD64水平明显升高,特别对早产儿感染诊断的敏感性和特异性均高于CRP、IL-6,可以作为诊断早产儿感染有力的实验室依据。

Objective：To evaluate the application of analyzing neutrophil cell surface marker CD64 in diagnosis of premature infants infection. Methods： 109 infants inpatient in neonatal department（ including NICU） were enrolled in the study. CD64 was measured by FCM, which was compared with C-reactive protein（CRP） and IL-6. Results：There was a statistically significant difference in quantitation of CD64 on neutrophil cells（P 〈0. 001 ） among the infected premature infants（5 010. 95 ±3 318.01 molecules/cell）, infected full-term infants （ 5 193.07 ± 3 207.32）, non-infected premature infants （2 277.80 ± 914. 56） as well as full-term infants （ 2 174. 34 ± 836.00）. The sensitivity, specificity, predictive values between positive and negative individuals for CD64 detection were very high, better than that for CRP, IL-6. Conclusion： CD64 levels are significantly elevated in newborns infected, compared with CRP, IL-6 assays, the sensitivity and specificity are all higher, particularly for the diagnosis of infection in premature infants. It can be used for laboratory diagnosis of neonatal infection effectively.