用细菌粘附法比较TC4钛合金及其表面改性后的抗菌能力

COMPARISON OF TC4 TITANIUM ALLOY AND MODIFIED SURFACES ANTIBACTERIAL PROPERTY BY BACTERIAL ADHESION METHOD

对牙种植体用材料TC4(Ti6Al4V)进行不同工艺的表面改性处理。应用细菌粘附方法,在荧光显微镜下直接计数,观察未处理试样和不同工艺处理后试样表面的细菌粘附,以评价改性表面的抗菌能力;同时采用SPSS统计软件分析对比两种工艺处理后改性表面的抗菌能力。细菌粘附和统计结果显示,未处理试样表面粘附了大量细菌;处理后试样表面粘附数量已显著减少;两种处理工艺改性后的表面之间细菌粘附量的差别没有统计学差异,即两种工艺处理后的改性表面的抗菌能力没有显著的差异。

Titanium alloy TCA （Ti6Al4V） used as dental implant materials were modified by different surface modification technologies. The bacterial adhesion method and the tagging effect of fluorescence were employed to evaluate antibacterial property of modified surfaces by observing bacterial adhesion status and counting quantities of bacterial adhesion on the Ti6Al4V surfaces below the fluorescence microscope; SPSS software was used to analysis and compare the antibacterial property of modified surfaces at the same time. The bacterial adhesion assay and statistic analysis results showed that： compared to the unmodified surface, both of ion nitriding-treated and ion plating-treated specimen surfaces could reduce bacterial adhesion significantly, and also there were no obvious differences between the two modification technologies treated surfaces at quantities of bacterial adhesion by statistic analysis, which means there were no obvious differences between the two modified processes treated surfaces at antibacterial property.