镧-甲基蓝光度法测定硫酸卡那霉素及硫酸新霉素

Spectrophotometric Determination of Kanamycin and Neomycin by Ion-association Reaction with the Binary Complex of La(Ⅲ) and Methyl Blue

镧（Ⅲ）-甲基蓝（MB）在酸性条件下与硫酸卡那霉素（KANA）及硫酸新霉素（NEO）反应生成蓝色离子缔合物,两者的最大正吸收波长均位于682 nm,表观摩尔吸光系数为1.71×10^4L·mol^-1·cm^-1（KANA）和1.21×10^4L.mol^-1·cm^-1（NEO）;最大负吸收波长位于620 nm（KANA）和616 nm（NEO）,表观摩尔吸光系数为9.57×104L.mol^-1.cm^-1（KANA）和8.68×10^4L.mol^-1.cm^-1（NEO）;用双波长法测定时,摩尔吸光系数可达1.10×105L.mol-1.cm-1（KANA）和1.00×10^5L.mol-1·cm^-1（NEO）。方法用于市售药物中硫酸卡那霉素及硫酸新霉素含量的测定,结果与标示值相符,回收率在99.8%~100.5%之间。

In an acetate buffer solution of pH 4.32, the binary complex formed by lanthanum（ Ⅲ） and methyl blue reacted with kanamycin （KANA） or neomycin （NEO） to form blue ion association complexes, having positive absorption maxima at 682 nm for both KANA and NEO, with molar absorptivity of 1.71 × 10^4 L · mol^-1 · cm^-1 （for KANA） and 1.21× 10^4L · mol^-1 · cm^-1 （for NEO）, and negative absorption maxima at 620 nm （for KANA） and 616 nm （for NEO）, with molar absorptivity of 9. 57 × 10^4 L ·mol^-1 · cm^-1 （for KANA） and 8. 68 × 10^4 L · mol^-1 · cm^-1 （for NEO）. When the absorption measurement was made by DW-spectrophotometry, the values of molar absorptivity acheved to 1.10 × 10^5 L · mol^-1 · cm^-1 （for KANA） and 1.00 × 10^2 L · mol^-1 · cm^- 1. In the analysis of a kanamycin injection sample and a neomycin ear drops sample, the results obtained were in consistency with the labelled values, and results of recovery tests were in the range of 99. 8%-100. 5%.