摘要
通过常规杂交，结合胚胎挽救方法，获得“泸州四棱油菜与海甘蓝的属间杂种，杂种的诱导率为２．１％。杂种Ｆ形态分别倾向于双亲之一保介于双亲之间，后期发育延迟，叶子皱缩。
An intergeneric hybrid between Brassica juncea (L.) Coss. x Crambe abyssinica Hochst. ex R. E. Fries was obtained for the first time through routine method combined with embryo rescue. The inductivity of hybrids was 2.1% . In many respects the F1 hybrid resembled either its maternal or paternal parent or both. Consequently, retarded growth, wrinkled leaves and rudimentary anthers in F1 plant were observed. F1 pollen varied in size and 10% ~ 15% were of the paternal pollen type,i.e. ,4-colpate. F1 pollen viability was low and 12% ~ 25% of pollens was viable , with a mean of 18.5 % . The chromosome number of F1 somatic cell was 63 which was intermediate between the two parents. Most of pollen mother cells had 63 chromosomes. Analysis of isozymes further confirmefl that the F1 hybrid combined the paternal characteristic bands.
基金
国家自然科学基金(39680026)
关键词
海甘蓝
芥菜型油菜
属间杂种
鉴定
Crambe abyssinica, Brassica juncea, Intergeneric hybrid, Identification
