HPLC-荧光法测定人血浆中替米沙坦血药浓度的方法改进

Improvement of HPLC-Fluorometric Method in the Determination of Telmisartan in Human Plasma

目的：改进以高效液相色谱-荧光法测定人血浆中替米沙坦浓度的方法.方法：以萘普生为内标,采用乙酸乙酯提取的方法处理血浆.色谱柱为Symmetry C8,流动相为乙腈-0.01mol·L^-1磷酸二氢钾溶液（47：53）,流速为1.0mL·min^-1荧光检测波长为305nm（激发波长）、365nm（发射波长）,进样量为20μL.结果：替米沙坦检测浓度在2.5～200ng·mL^-1范围内线性关系良好（r=0.999 8）.最低检测限为1ng·mL^-1.方法的提取回收率为（89.91±10.07）%,日内和日间RSD＜10%.样品3次冻融及提取后在24h内稳定性良好.结论：本方法操作简便、灵敏度和精确度高、重现性好,适于替米沙坦的体内药物浓度测定和药动学研究.

OBJECTIVE： To improve the HPLC-fluorometrie method in the determination of telmisartan in human plasma.METHODS： Using naproxen as internal standard, the plasma was extracted with ethyl acetate.The separation was performed on Symmetry Ca with mobile phase consisted of aeetonitrile -0.01tool · L^ -1 potassium dihydrogen phosphate buffer solution（47 ： 53） at a flow rate of 1.0ml · min^ -1.The λEx was set at 305nm and the ）,Era was set at 365nm, and the sample size was 20μL.RESULTS：The linear range of telmisartan was 2.5--200ng · mL^ -1（ r = 0.999 8） ,with the lowest deteetable limit at 1ng · mL^ -1.The average reeovery of extraetion was （89.91 ＋ 10.07） % .Both the intra - day RSD and inter - day RSD were less than 10% .The sample showed a good stability within 24 hours after three freeze- thaw cycles and extractions.CONCLUSI- ON：The method is simple, sensitive,accurate and reproducible,and suitable for the determination and pharmaeokinetie study of telmisartan in human plasma.