摘要
目的:改进以高效液相色谱-荧光法测定人血浆中替米沙坦浓度的方法.方法:以萘普生为内标,采用乙酸乙酯提取的方法处理血浆.色谱柱为Symmetry C8,流动相为乙腈-0.01mol·L^-1磷酸二氢钾溶液(47:53),流速为1.0mL·min^-1荧光检测波长为305nm(激发波长)、365nm(发射波长),进样量为20μL.结果:替米沙坦检测浓度在2.5~200ng·mL^-1范围内线性关系良好(r=0.999 8).最低检测限为1ng·mL^-1.方法的提取回收率为(89.91±10.07)%,日内和日间RSD<10%.样品3次冻融及提取后在24h内稳定性良好.结论:本方法操作简便、灵敏度和精确度高、重现性好,适于替米沙坦的体内药物浓度测定和药动学研究.
OBJECTIVE: To improve the HPLC-fluorometrie method in the determination of telmisartan in human plasma.METHODS: Using naproxen as internal standard, the plasma was extracted with ethyl acetate.The separation was performed on Symmetry Ca with mobile phase consisted of aeetonitrile -0.01tool · L^ -1 potassium dihydrogen phosphate buffer solution(47 : 53) at a flow rate of 1.0ml · min^ -1.The λEx was set at 305nm and the ),Era was set at 365nm, and the sample size was 20μL.RESULTS:The linear range of telmisartan was 2.5--200ng · mL^ -1( r = 0.999 8) ,with the lowest deteetable limit at 1ng · mL^ -1.The average reeovery of extraetion was (89.91 + 10.07) % .Both the intra - day RSD and inter - day RSD were less than 10% .The sample showed a good stability within 24 hours after three freeze- thaw cycles and extractions.CONCLUSI- ON:The method is simple, sensitive,accurate and reproducible,and suitable for the determination and pharmaeokinetie study of telmisartan in human plasma.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第20期1556-1558,共3页
China Pharmacy