摘要
利用JQ－700型高速基因枪将pDM302质粒DNA上的bar基因即PAT酶（phosphinothricinacetyltransferase）基因导入了冬小麦品种“农大146”的幼胚中。经过在含有phosphinothricin（简称ppt）的选择培养基上筛选，得到了9块具有ppt抗性的愈伤组织。PCR电泳检测与PCR－Southern杂交结果显示，外源bar基因已转化进了由其中4块愈伤组织再生出的转基因的小麦植株中。
The bar gene (phosphinothricin acetyltransferase gene) in plasmid pDM302 hadbeen transferred into the immature embryos of a winter wheat cultivar (Triticum aestivum L.) 'Nong Da 146' by microprojectile bombardment with JQ-700 system.Afterselection for 2-3 months,we have obtained 9 pieces ppt (phosphinothricin)-resistant calluses.PCR electrophoresis assay and PCR-Southern hybridization showed the foreignbar gene had been stably transformed into the genomes of the transgenic wheat plantletsregenerated from 4 of the 9 ppt-resistant calluses.
出处
《西北植物学报》
CAS
CSCD
1997年第2期142-146,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
"863"高科技项目资助
关键词
小麦
BAR基因
基因枪
转基因植株
wheat, bar, immature embryo, microprojectile bombardment, transgenic plant
