干旱对小麦幼苗诱导蛋白表达与某些生理特性的初步探讨

INITIAL STUDYS OF RELATIONSHIP BETWEEN THE APPEARANCE OF INDUCED-PROTEIN AND SOME PHYSIOLOGICAL CHARACTERS OF WHEAT SEEDLING IN WATER STRESS

试验以－1．2MPaPEG6000处理萌动小麦种子（TriticumaestivumL．）。SDS－PAGE图谱分析表明，水分胁迫诱导幼芽及整株均产生48．4kD、41．5kD二个蛋白质亚基。在幼根中，未出现以上二个蛋白亚基。胁迫48h后，根干重／芽干重比呈上升趋势，幼芽细胞膜相对透性增大和相对含水量下降幅度均大于幼根。复水48h，幼芽迅速恢复生长，表现出一个补偿性生长阶段，而主胚根部分死亡，随后，又重新从根鞘中发出新根。再胁迫时，幼根细胞膜相对透性增大和相对含水量下降幅度均大于幼芽，此时，根干重／芽干重比呈下降趋势，表明脱水能存活幼芽组织和诱导蛋白积累之间具有一定的相关性。

Changes in protein profiles induced by water stress (-1. 2MPa PEG) were investigated in roots and shoot of germinating seeds of wheat.As revealed by SDS-PAGE,Two new proteins having molecular weights of 48. 4kD and 41.5kD, accumulated onlyin shoot and seedling.The two new proteins did not accumulated in roots. 48h aftertreatment with PEG at - 1. 2MPa, the degrees of the inhibition growth of shoots wasgreater than that the roots,and the degrees of the decrease of RWC and the increase ofmembrane permeability in shoots were higher than the roots. When water stressseedling were rewatered,shoot quickly resumed growth. The roots were partly died.Newroots developed later from the root sheath tissue. The degrees of the decrease of RWCand the increase of membrane permeability in roots were higher than the shoot when retreat.The results indicated that there is a correlation between dehydration survival andinduced protein accumulation.