湘江野鲤养殖群体和自然群体遗传多样性的微卫星分析

Microsatellite DNA analysis on genetic diversity of cultured and wild Cyprinus carpio populations in Xiangjiang River

采用微卫星技术,用17对微卫星引物对湘江野鲤养殖群体和自然群体的的遗传多样性进行分析。结果表明:有15对引物扩增出清晰的条带,其中13对引物在群体间呈现多态性;2个群体中,13对多态性引物分别扩增等位基因2～12个,共90个,其中35个等位基因为2群体共有,55个等位基因具有群体特异性,引物平均等位基因数为6·92个,等位基因频率为0·0667～0·8333;养殖群体和自然群体的平均遗传杂合度和平均多态信息含量分别为0·5688、0·5152,0·5860、0·5347;2个群体间遗传相似性指数为0·6762,遗传距离为0·3238,表明湘江野鲤养殖和自然群体遗传多样性均较为丰富,2个群体间遗传变异程度较高。

By using microsatellite technology and 17 pairs of microsatellite DNA primers, the genetic diversities of cultured and wild Cyprinus carpio populations in Xiangjiang River were studied. 15 of the 17 pairs of microsatellite primers were amplified with clear bands, among which, 13 pairs presented polymorphism. 2-12 alleles per pair in the two populations were amplified, with 35 alleles coexisted in both of the two populations and 55 alleles in each population. The average number of the alleles per pair was 6. 92, and the frequency of the alleles was 0. 0667-0. 8333. The average heterozygosity and polymorphism information contents of cultured and wild C. carpio populations were 0. 5688 and 0. 5152, and 0. 5860 and 0. 5347, and the genetic similarity index and genetic distance were O. 6762 and O. 3238, respectively, indicating that the genetic diversity of the two populations was relatively abundance, and the genetic variability was high.