土壤羟胺还原酶活性测定方法的改进

A modified method for measuring soil hydroxylamine reductase activity

对传统土壤羟胺还原酶活性的测定方法进行了部分改进。用煮沸并密封冷却的蒸馏水形成3～5cm的液封,同时用N2气流排除液面以上的空气,能够创造测定土壤中羟胺还原酶活性所需要的厌氧环境。与传统方法相比,该方法减少了厌氧程度的不确定性和试验步骤的复杂性,增加了培养试验的可操作性。以碘量法为对照,对4种不同的测量浸提液中羟胺浓度的分光光度法进行了对比筛选,结果表明,硫酸铁铵-邻菲罗啉法具有较高的准确度和精密度,是测定土壤中羟胺还原酶活性的理想方法。

In this paper, the traditional measuring method of soil hydroxylamine reductase activity was partially modified. The anaerobic condition needed for the measurement was created by a 3 -5 cm fluid seal, which was made from the boiled water having been cooled down under airproof condition. The air above the fluid seal was exhausted with N2 current to prevent O2 dissolving into water. Compared with traditional methods, this method could reduce the uncertainty and the complexity in operation, and increase the maneuverability. Taking iodometric analysis as the reference, four spectrophotometric methods were adopted to determine the hydroxylamine concentration in extract, and the results showed that ammonium ferric sulfate-phenanthroline method was of high accuracy and precision, being feasible in measuring soil hydroxylamine reductase activity.