应用细胞工程获得受主效基因控制的水稻耐盐突变系

Salt-Tolerance Rice Mutant Lines Controlled by a Major Effect Gene Were Obtained by Cell Engineering Technique

实验以水稻花药为材料，经ＥＭＳ处理或未处理，在ＮａＣｌ胁迫下筛选耐盐突变体。所获变异系耐盐性已稳定遗传到第１３代。回交测定Ｆ２耐盐性呈３∶１的分离，表明耐盐性受一个主效基因控制。实验结果还表明，通过合适的筛选方法，无论诱变处理与否，都能得到稳定遗传的耐盐突变体。

nthers of rice (Oryza sativa) treated or untreated with ethylmethanesulphonate (EMS) were used to select the NaCl-tolerant calli on N6 agar medium containing 0.5%,0.8% and 1.0% NaCl respectively.Three NaCl-tolerant mutant lines (M1,M2 and M3) were obtained from 22 fertile R1 plants after selection in soil containing 0.5% NaCl.Their salt-tolerant trait have been stably inherited for thirteen generations.In the F2 populations of the hybrids derived from the mutants backcrossed with their parent,one forth plants fell dead in soil containing 0.5% NaCl,three fourths plants seeded normally.This result suggested that the effect of a major gene may be presented in these mutants.