1Centola GM, Herko R, Andolina E, et al. Comparationof sperm separation methods effect on recovery,motility, motion parameters, and hyperactivation. Fertil Steril 1998; 70(6): 1173-1175
2Claassens OE, Menkveld R, Harrison KL. Evaluation substitutes for Percoll in sperm isolation by density centrifugation. Hum Reprod 1998; 13:3139-3143
3Ostling O, Johanson KL. Microelectrophoretic study of radiation-induced DNA damages in individual cells.Biochem Biophys Res Commun 1984; 123:291-298
4Singh NP, McCoy MT, Tice RR, et al. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175:184-191
5Hughes CM, Lewis SE, McKelvey-Martin V J, et al.The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity.Hum Reprod 1998; 13(5):1240-1247 Erratum in:Hum Reprod 1998; 13(11): 3284
6Zini A, Nam RK, Mak V, et al. Influence of initial semen quality on the integrity of human sperm DNA following semen processing. Fertil Steril2000; 74:824-827
7Donnelly ET, McClure N, Lewis SE. Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity. Fertil Steril 2001; 76(5):892-900
8Menkveld R, Swanson RJ, Kotze TJ, et al. Comparison of a discontinuous Percoll gradient method versus a swim-up method: effects on sperm morphology and other semen parameters. Andrologia 1990; 22(2): 152-158
9Stanic P, Tandara M, Sonicki Z, et al. Comparison of protective media and freezing techniques for cryopreservation of human semen. Eur J Obstet Gynecol Reprod Biol 2000; 91 (1): 65-70
10Soderlund B, Lundin K. The use of silae-coated silica particles for density gradient centrifugation in in-vitro fertilization, Hum Reprod 2000; 15(4): 857-860
10Li Z, Zhou Y, Liu R, et al.Effects of semen processing on the generation of reactive oxygen species and mitochondrial membrane potential of human spermatozoa[J].Andrologia, 2012, 44 (3) : 157-163.