氯胺酮抑制内毒素休克大鼠中性粒细胞的CD11b表达和呼吸氧爆发

Ketamine inhibits CD11b expression and respiratory burst activity of polymorphonuclear leucocytes in endotoxemia rats

目的观察氯胺酮对内毒素休克大鼠中性粒细胞CD11b表达和呼吸氧爆发的影响。方法腹腔内注射内毒素(10mg/kg)复制脓毒症模型。30只成年雄性SD大鼠,随机分为5组:生理盐水组;内毒素刺激组;内毒素刺激+氯胺酮(5mg.kg-1.h-1)治疗组;内毒素刺激+氯胺酮(25mg.kg-1.h-1)治疗组;内毒素刺激+氯胺酮(50mg.kg-1.h-1)治疗组。在1、4、6h使用流式细胞仪检测中性粒细胞CD11b和呼吸氧爆发。结果与生理盐水组相比内毒素刺激后中性粒细胞的CD11b的表达和呼吸氧爆发明显增加(P<0.01)。与单纯内毒素刺激组相比,使用氯胺酮治疗组CD11b表达和呼吸氧爆发明显减少(P<0.01)。结论氯胺酮抑制内毒素休克大鼠中性粒细胞的CD11b表达和呼吸氧爆发。

Objective To observe the effect of ketamine on polymorphonuclear leucocytes （PMN） adhesion and respiratory burst acticity in endotoxemia rats. Methods Thirty SD rats were randomly allocated to five groups： rats challenged with intraperitoneal injection of saline （saline group）, challenged with intraperitoneal injection of lipopolysaccharide （LPS） 10 mg/kg （LPS group）, challenged with intraperitoneal injection of LPS 10 mg/kg and treated by intraperitoneal injection of ketamine 5, 25, 50 mg · kg^-1 · h^-1 , respectively （K05, K25, and k50 groups）. PMN respiratory burst and CDllh expression were measured with flow cytometry at 1, 4, and 6 h. Results LPS challenge significantly increased PMN respiratory burst activity and CDllh expression when compared with the saline group （P〈0.01）. There was a significant decrease in LPS-induced PMN respiratory burst activity and CDllh expression in three ketamine treatment groups when compared with LPS group （P 〈 0.01 ）. Conclusion Ketamine significantly inhibits PMN CD11h expression and respiratory burst activity in endotoxemia rats.