石斛属植物遗传多样性RAPD分析

Analysis and Classification of Dendrobium Species (Orchidaceae) Plants in China by RAPD

应用随机扩增多态性DNA(RAPD)技术,对石斛属植物16个种进行了基因组DNA多态性分析。从82个随机引物中筛选出20个多态性好的引物进行RAPD扩增,扩增DNA片段数据用UPGMA聚类法构建系统发育树。20条引物共扩增出142条带,多态性带118条,约占总数的83.1%。聚类结果显示RAPD分子标记构建的系统发育树与经典分类系统一致。RAPD标记可为石斛属植物的系统分类研究提供分子生物学依据。

RAPD was employed to probe genetic differences of 16 species of Dendrobium. 20 primers with good polymorphism screened from 82 random primers were used to conduct RAPD analysis and dimorphic data of DNA f ragments were used to construct the dendrogram by UPGMA. Totally 142 bands were amplified and 118 of them were polymorphic accounting for 83. 1%. Clustering results revealed that the dendrogram constructed with RAPD molecular makers was identical with the classic taxonomic system. Dendrobium plant are rich in genetic diversity and different populations in the same species vary much in genetic differentiation. The RAPD markers can provide the basis for taxonomic researches of Dendrobium plants.