金脉单药花组培工厂化技术研究

Study on the Industrialized Rapid Clonal Propagation Technology of Aphelandra squarrosa cv "Dania"

选用金脉单药花健壮枝条作为材料，进行组培工厂化育苗繁殖技术研究。结果表明：愈伤组织诱导激素组合以6-BA0．5mg／L＋NAA0．2mg／L最为适合，愈伤组织的生长速度和质量均满足要求，继续培养可分化产生大量不定芽；继代培养采用6-BA0．3mg／L＋NAA0．1mg／L，增殖倍率控制在6～8倍；生根培养采用NAA0．5mg／L＋IBA0．1mg／L，一般15d左右生根，平均根量5～6条，生根率100％，移栽成活率高，满足植物组织培养工厂化育苗技术要求。

Selects the vigorous and healthy branch of Aphelanclra squarrosa cv“Dania” as explants carried on the industrialized rapid clonal propagation technology research. The results showed that plant hormone mmbination of 6-BA0. 5mg/LA-NAA 0. 2mg/L is best for callus induction and Adventitious buds differentiation. 6-BA 0. 3mg/L ＋ NAA0. 1mg/L is suitable for continuous cultivation,the coeffident of reproduction is up to 6～8. NAA 0. 5mg/L＋IBA 0. 1mg/L is good for rootage,rooring rate was 100% ,and completely meets the demands of the industrialized rapid clonal propagation technology.