彩叶草组织培养研究

Study on the Plant tissue culture in Coleus blumei Benth.

以唇形科鞘蕊花属彩叶草（Coleus blumei Benth）叶片为外植体，进行组织快繁的研究。结果表明：叶片组织培养中，最适宜的消毒处理为消毒剂采用0．1％升汞溶液，加入3～4滴吐温80，消毒时间为5min，总体污染率低于20％；最适的愈伤组织诱导及继代培养基为1／2MS＋2．0mg／L6-BA＋1．0mg／LNAA的组合，其平均诱导率为72％；最适分化培养基是1／2 MS＋1．0mg／L 6-BA＋0．5mg／LNAA的组合，其平均分化率为85．4％；彩叶草不定芽的最适生根培养基是1／2MS＋0．5mg／LNAA组合，其平均生根率为92．5％。

The study of the plant tissue culture constructed rapid propagation system of Coleus blumei Benth. with its leaves as the explants. From this research, the main results were as follows： In the investigation of plant tissue culture with leaves of Coleus blumei Benth. , the optimum disinfection method was using 0. 1% solution of mercuric chloride as the disinfectant with 3-4 drop of Tween-80, and the disinfection last 5 min. The rate of pollution with this method was below 20%. The optimum culture medium of inducement callus and sub culture was 1/2MS＋2. 0 rng/L 6-BA＋1.0 rng/L NAA, and its average rate of inducement was 72%. The optimum culture medium of differentiation culture was 1/2MS＋1.0 mg/L 6-BA＋0. 5 rng/L NAA, and its average rate of differentiation was 85. 4%. The optimum culture medium of rooting culture was 1/2MS＋0. 5 mg/L NAA,and its average rate of rooting was 92. 5%.