p38 MAPK信号通路参与受体介导的细胞内吞的调控

p38 MAPK signaling pathway is involved in the regulation of receptor-mediated endocytosis

目的:观察p38MAPK信号转导通路在受体介导的细胞内吞中的作用。方法:利用Alexa594标记的转铁蛋白作为受体介导的内吞作用的观察指标,来研究p38特异性抑制剂SB203580或ERK通路特异性抑制剂PD98059预处理以及p38基因敲除对该过程的影响。结果:在受体介导的细胞内吞中,p38被磷酸化激活。SB203580的预处理或p38基因的敲除都能阻断受体介导的细胞内吞,而PD98059的预处理对该过程没有影响。结论:p38MAPK信号转导通路参与了受体介导的细胞内吞的调控。

AIM ： To investigate the role of p38 MAPK signaling pathway in the receptor - mediated endocytosis. METHODS： The effects of p38 MAPK on the receptor - mediated endocytosis were observed by using Alexa 594 - conjugated Transferrin, in the presence of p38 specific inhibitor SB203580 or ERK pathway specific inhibitor PD98059, or using p38 knockout techniques. RESULTS ： In the process of receptor - mediated endocytosis, p38 was activated by phosphorylation. Furthermore, the receptor - mediated endocytosis was inhibited by pretreatment with SB203580 or p38 knockout, while pretreatment with PD98059 had no effect on this process. CONCLUSION： p38 MAPK signaling pathway plays a role in the regulation of receptor - mediated endocytosis.