导数-同步荧光光谱法测定尿液肾上腺素和去甲肾上腺素

Simultaneous determinaation of epinephrine an dnorepinephrine in urine By Derivative Synchronous Fluorescence Spectroscopy

为了解决肾上腺素（Ｅ）、去甲肾上腺素（ＮＥ）氧化产物的荧光光谱严重重叠难题，将同步荧光与导数光谱两种技术结合起来用于尿液Ｅ、ＮＥ测定。结果发现，在激发波长与发射波长相差Δλ＝４０ｎｍ时获得的Ｅ、ＮＥ二阶导数－同步荧光光谱中，４５０ｎｍ处Ｅ的荧光强度的导数信号不受ＮＥ的干扰；４３２ｎｍ处，ＮＥ的荧光强度的导数信号不受Ｅ的干扰，Ｅ、ＮＥ含量在１～２００ｎｇ／ｍｌ范围内成线性关系；最低检测限为０．５ｎｇ／ｍｌ；批内ＣＶ３．２１％～３．９５％，批间ＣＶ４．２６％～５．１０％，Ｅ和ＮＥ的回收率分别为９４．９％～９５．４％、９４．７％～９６．３％；该法操作简单、快速、成本低廉，适合于％批量测定，是测定尿液Ｅ、ＮＥ较为理想的常规方法。

so as measured epinephrine and norepinephrine in urine by combing both techniques of synchronous and derivative fluorimetry. Were measured, to resolve difficulty of oxidation product of epinephrine and norepinephrine that present strongly overlapping conventrational fluorescence spectra. The cxperimental result shows that the lowest detectable concentration of both epinephrine and norepinephrin are below the level of 0 5 ng/ml. The linear range is 1 to 200 ng/ml for epinephrine and norepinephrine. Analytical recovery is 94 9% to 95 4% for epinephrine, 94 7%～96 3% for norepinephrine. The intra and interassay CV were 3 31% to 3 95% and 4 26% to 5 10%, respectively. The method presents a simple, rapid, precise and lower cost. It is satisfying for routing measurement epinephrine and norepinephrine in urine.