增强化学发光免疫法检测乙型肝炎表面抗原的建立和评价

Evaluation of method by enhanced luminescence immunoassay to detect surface antigen of hepatitis B virus

本法采用Ｌｕｍｉｎｏｌ－Ｈ２Ｏ２－ＨＲＰ系统建立了增强化学发光免疫法（ＥＬＥＩＡ）检测乙型肝炎表面抗原（ＨＢｓＡｇ），该发光信号的强度与血清中ＨＢｓＡｇ浓度呈线性关系，回归方程为：Ｙ＝０．４４４１Ｘ＋０．００６７，相关系数ｒ＝０．９９７１；批内变异系数为３．６％～９．３％、批间变异系数为１０．２％～１２．５％；回收率为８２．５％～１１５．０％；最小检测限为２０ｐｇ／ｍｌ；结果表明本法有灵敏性高，准确性和稳定性好的特点。

By utilizing luminol H 2O 2 HRP system, a enhanced luminescence immunoassay (ELEIA) method to measure HBsAg serum concentration was established。There is a good correlation between strength of light emission and serum concentration of HBsAg (r=0 9971)。The CV of within and without ran was 3 6%～9 3%(n=20),10 2%～12 5% (n=7) respectively, the recovery was 82 5%～115 0%。The detection limit was 20 pg/ml。ELEIA is a sensitive, acurate and stable method and is used to clinic routine work。