BCP/HAFG-rhBMP-2复合人工骨修复骨缺损的实验研究

Experimental study of BCP/HAFG-rhBMP-2 artificial bone for the repair of limb defect

目的研制理想的能较快修复大段骨缺损的人工骨材料。方法将重组骨形态发生蛋白-2(rhBMP-2)/透明质酸钠-纤维蛋白复合凝胶(HAFG)缓释体系和多孔双相钙磷陶瓷(BCP)结合研制成BCP/HAFG-rhBMP-2人工骨,并分别将BCP/HAFG-rhBMP-2、BCP/rhBMP-2和BCP/HAFG人工骨进行兔桡骨大段骨缺损修复的对比研究。术后分别行大体、放射学、组织形态学及生物力学测试。结果试验中第12周与第2周相比较,3组碱性磷酸酶(AKP)值下降情况分别为BPC/HAFG-rhBMP-2组降低了65.13%,BPC/BMP-2组降低了71.03%,BPC/HAFG组降低了58.59%。组织学及扫描电镜观察显示:12周时BPC/HAFG-rhBMP-2组材料已基本被成熟骨组织所代替,新骨结构与宿主骨无差别,而BPC/HAFG和BPC/rhBMP-2组骨小梁结构疏松细小,材料仍未完全被降解,材料与骨组织间形成较大间隙。术后12周时3组材料植入部位骨组织极限抗压强度分别为:BCP/HAFG-rhBMP-2组(538±12.7)N、BCP/BMP-2组(368±24.0)N、BCP/HAFG组(256±8.4)N。BPC/HAFG-BMP-2复合型人工骨较BCP/BMP-2及BCP/HAFG人工骨具有更强、更持久的诱导成骨活性。结论BCP/HAFG-rhBMP-2人工骨能更快促进长骨大段骨缺损的修复,是一种较理想的人工骨材料。

Objective To design an ideal artifical bone material for rapid repair of segmental defect of long bones. Methods Recombinant human bone morphogenetic protein-2 （rhBMP-2 ） loaded compound gel of fibrin glue（FG） and sodium hyaluronate（HA） and porous biphasic calcium phosphate（BCP） were combined with BCP/ HAFG-rhBMP-2 artificial bone and then compared with BCP/ rhBMP-2 and BCP/ HAFG artifical bones on the prosthetic study of stage defect of rabbit radius. Specimens were procured at 2,4,8 and 12 weeks after operation and gross observation,histological examination, biomechanics examination and imageology investigation were performed. Results From the second week to the 12 th week, the respective descended ratio of the AkP activity in three groups were 58.59% in BPC/HAFG group,65.13% in BPC/HAFG-rhBMP-2 group and 71.03% in BPC/BMP-2 group. At the 12 weeks after operation, the histologically analyses showed that the BCP/ HAFG-rhBMP-2 artificial bone was replaced by the new ripe bones, but the bone trabeculas were crumbly and leptos ,and some artificial bones still existed in BPC/BMP-2 and BPC/HAFG groups. The respective maximum compressive strength in three groups were （ 538 ± 12.7 ） N in BCP/ HAFG- rhBMP- 2 group, （ 368±24.0 ）N in BCP/BMP-2 group and （256 ± 8.4 ）N in BCP/ HAFG group at the 12 weeks after operation. All resuits showed the BCP/ HAFG-rhBMP-2 artificial bone possesses stronger and longer osteoinductive activity than BCP/ rhBMP-2 and BCP/ HAFG artifical bones in each time point Conclusion The BCP/ HAFG-rhBMP-2 artificial bone is able to promote early repair of long bone defect and is an ideal artificial bone.