Ru(phen)_2 dppx^(2+)光谱探针法研究道诺霉素与脱氧核糖核酸的相互作用

Interaction Between Daunomycin and Deoxyribonucleic Acid Using Ru(phen)_2 dppx^(2+) as Spectroscopic Probe

以核酸分子“光开关”Ru(phen)2dppx2+为探针,分别采用紫外-可见吸收光谱法和荧光光谱法研究了抗癌药物道诺霉素(daunomycin,DNM)与小牛胸腺DNA之间的作用方式,结果表明:DNM是以插入的方式与DNA相互作用。Scatchard方程的研究表明,Ru(phen)2dppx2+与小牛胸腺DNA的结合比为1∶4~1∶5;表观结合常数为2.58×107L/mol。DNM加入后,表观结合常数大大降低,这也表明道诺霉素主要是以插入方式与DNA结合。作为研究核酸分子的荧光探针,与溴化乙锭相比,Ru(phen)2dppx2+具有灵敏度高、毒性低、稳定性好、选择性好、使用方便等优点。

The interaction between daunomycin （DNM） and ctDNA was studied using { Ru（ 1,10-phenanth- roline ） 2 7,8-dimethyl-dipyrido [ 3,2-a： 2＇, 3＇-c ] phenazine ） }^2＋ （ Ru （ phen ） 2 dppx^2＋ ） as an interacting mode spectroscopic probe. The way for Ru （phen）2 dppx^2＋ action on DNA is intercalation. The study of the influence of DNM on the ultraviolet spectra and fluorescence spectra of Ru （phen）2 dppx^2＋ -DNA system indicates that DNM intercalates the base pairs of DNA. The study of the Scatchard equation shows that the apparent binding constant between Ru （phen）2 dppx^2＋ and DNA is 2.58 × 10^7 L/mol, and the binding ratio is about 1：4 -1：5. The apparent binding constant decreases dramatically while DNM was added, which indicates that DNM intercalates the base pairs of DNA. Comparing with normal DNA interacting mode spectroscopic probe Ethidium Bromide （EB）, Ru（phen）2 dppx^2＋ has the advantages of higher sensitivity, lower toxicity, higher stability and simplicity.