自制微透析探针性能评价

Performance Evaluation of Self-made Microdialysis Probes

以自制微透析探针为研究对象,通过体外芍药苷微透析实验,评估灌注液流速、人工细胞外液中药物浓度、回收率测定方法及半透膜性质对自制同轴型微透析探针取样回收率的影响。实验结果表明,自制探针的回收率随灌注液流速升高呈近似指数曲线降低。当灌注流速为8.0μL/min时,回收率表现出浓度依赖性。采用直接透析法或反向透析法所得同一探针回收率存在显著差异。回收率因探针不同而存在差异。探针在体内连续取样6h,半透膜的通量未见衰减。实验室自制探针性能稳定,可用于体内微透析研究。

With peoniflorin used as a model compound, in vitro microdialysis experiments were carried out to evaluate the influence of a series of experimental conditions （perfusate flow rate, concentration of peoniflorin, membrane and method used to determine recovery） on the extraction efficiency of self-made concentric probes： The results revealed that extraction efficiency was different between probes. An exponential decrease in extraction efficiency parallel to the increase in perfusate flow rate was found. Extraction efficiency became concentration-dependent when the flow rate was 8.0 μL/min. Statistically significant differences was found between extraction efficiency determined by direct dialysis and retrodialysis. After the self-made probe was applied to continuously sampling in vivo for 6 hours, the permeability of the membrane on the probe did not significantly. The results show that the self-made microdialysis probes can be used for microdialysis in vivo.