补肾化瘀汤对慢性再生障碍性贫血患者骨髓细胞嗜银蛋白的影响

Effect of “Bushen Huayu Decoction” on Ag-NORs of Patients with Chronic Aplastic Anemia

运用计算机图像分析技术,对61例慢性再生障碍性贫血患者(CAA)经补肾化瘀汤治疗前后,与61例健康人骨髓细胞核仁组成区相关的嗜银蛋白(AgNORs)进行每个核含量和颗粒总数的测试分析。结果:CAA患者治疗前骨髓细胞平均每核AgNORs含量和颗粒总数明显低于正常对照(p<0.01),治疗后与健康人趋于同一水平。补肾化瘀汤影响骨髓细胞Ag-NORs的机理可能与改善骨髓微循环或刺激造血细胞从而促进rDNA或rRNA的合成代谢有关。本研究为临床应用补肾化瘀治疗CAA提供了部分实验依据。

Determination and analysis on the every nucleus of content and that of sum total in Ag-NORs from myeloid cell nuclues in 61 cases healthy individuals and in 61 cases patients with chronic aplastic anemia (CAA) pre-and post-treatmentally with Bushen Huayu Decoction, respectively, were performed with MPV-TA microspeotroscopic image analyser. The results showed the average content of every nuclues Ag-NORs and total of granule were significantly lower than that of control (p<0. 01) prior to the treatment and raised to the same level approximately as that of after the treatment. Mechanism of the 'Bushen Huayu Decoction' effect myeloid cell Ag-NORs might result from the improvement of microcirculation or/and stimulus to hematopoietical cell which accelerated the rDNA or rRNA synthesis and anabolism in these cells. This study provided section experiment evidence for treatment CAA with Bushen Huayu Decoction in the clinical use.