摘要
针对核桃茎尖初代培养易褐变的问题,以3个核桃品种为试材,研究了预处理和培养基中添加抗氧化剂和吸附剂对褐变程度的影响。结果表明:经1～2℃冷藏处理24 h,20%的Na2S2O3浸泡20～30 min,流水冲洗8～10 h并在培养基中添加2.0 g/L PVP的处理,在短期内可有效地防止3个品种的褐变。
Three walnut cultivars were used as experiment materials, different methods was used to pretreatment of walnut explants and added antioxidants or adsorbents to primary medium to study on the walnut shoot tips browning. The result showed that ： the effective method to anti-browning for three cultivars in short-term was that shoot tips of walnut was pretreatment under cold storage at 1-2 ℃ for 24 h; then dipped in the 20%sodium thiosulfater for 20-30 minutes and subsequently washed with tap water for 8-10 hours ,and then, cultured in the medium added with PVP 2.0 g/L.
出处
《北京农学院学报》
2007年第2期17-19,共3页
Journal of Beijing University of Agriculture
基金
国家科技部基础工作资助项目:核桃
板栗资源圃标准化整理
整合及共享试点(2006DKA21002-20)
北京市教委都市农业学科群资助项目(XK100190553)
关键词
核桃
茎尖
离体培养
褐变
品种
Juglans regia
shoot tips
in vitro culture
browning
cultivar
