牙周炎龈组织中IL-1α基因的表达

The Expression of IL-1α Gene in Gingival Tissue

白细胞介素 1(IL- 1)是由多种细胞分泌的一种免疫调节因子 ,在炎症过程中可介导多种病理损害。本实验通过反转录 -聚合酶链反应 (reverse transcription- polym erase chain reaction,RT- PCR)技术检测龈组织中 IL- 1α基因的表达 ,初步探讨 IL- 1α与牙周炎发病机理的关系。结果表明 ,5例牙周炎龈组织中均能检测到 IL- 1α基因片段的表达 ,而在 2例正常龈和 3例治疗后龈组织中均未能扩增到 IL - 1α基因片段。结果提示 ,IL - 1α在牙周炎时有较显著的表达 ,是牙周炎时较特异的细胞因子 。

IL 1 is secreted by a variety of cells and plays an important role in the network of cytokines. In this study, the expression of IL 1α in gingival tissue was determined with reverse transcription polymerase chain reaction (RT PCR). The PCR product was confirmed by DNA sequencing. The results showed that IL 1α transcription was detected in all 5 inflammatory gingival tissue samples, but it was not delected in the normal gingival tissue samples and in the samples after treatment. These findings suggested that IL 1α may be a specific indicator of periodontal disease, and a potential mediator in pathogenesis of periodontitis.