变形链球菌表面蛋白P1粘膜免疫动物模型的建立及霍乱毒素的免疫增强作用

An Animal Model for the Mucosal Immunization with\; the Surface Protein Antigen P1 from Streptococcus mutans and Immune Enhancement of Cholera Toxin

用共价偶联的方法 ,将变形链球菌表面蛋白 P1与霍乱毒素 B亚单位或前霍乱原类毒素进行偶联后 ,用灌胃的方法将不同的免疫剂进行大鼠免疫实验 ,检查不同时间大鼠体内特异抗体的产生情况。结果证实了霍乱毒素 B亚单位的佐剂效果 ,同时发现前霍乱原类毒素具有强的胃肠免疫增强作用。

To study the immune enhancement of cholera toxin by developing an mucosal immune animal model, SD rats were immunized using different antigen composation through intragastric route (I.G). The results showed that the effects of immunization became stronger after P1 was conjugated to cholera toxin B subunit (CTB) or procholeragenoid (PCG). Even μg dose of P1 could induce the rising of anti P1 SIgA antibodies in the rat's saliva, but the antibodies risied little without this adjuvant. These implyed that the conjugation was much necessary in IG immunization. The immune enhancement of PCG was greater than that of CTB.\;