摘要
用共价偶联的方法 ,将变形链球菌表面蛋白 P1与霍乱毒素 B亚单位或前霍乱原类毒素进行偶联后 ,用灌胃的方法将不同的免疫剂进行大鼠免疫实验 ,检查不同时间大鼠体内特异抗体的产生情况。结果证实了霍乱毒素 B亚单位的佐剂效果 ,同时发现前霍乱原类毒素具有强的胃肠免疫增强作用。
To study the immune enhancement of cholera toxin by developing an mucosal immune animal model, SD rats were immunized using different antigen composation through intragastric route (I.G). The results showed that the effects of immunization became stronger after P1 was conjugated to cholera toxin B subunit (CTB) or procholeragenoid (PCG). Even μg dose of P1 could induce the rising of anti P1 SIgA antibodies in the rat's saliva, but the antibodies risied little without this adjuvant. These implyed that the conjugation was much necessary in IG immunization. The immune enhancement of PCG was greater than that of CTB.\;
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1997年第1期51-54,共4页
West China Journal of Stomatology
基金
国家教委博士点科研基金资助
关键词
变形链球菌
表面蛋白
粘膜免疫
动物模型
龋齿
mucosal immunization\ \ procholeragenoid (PCG)\ \ intragastric route (IG)