粘性放线菌对牙面粘附机理的研究Ⅳ.粘性放线菌唾液蛋白受体的研究

The Study of Actinomyces viscosus Adhere to Teeth \;Ⅳ. The Study of Salivary Protein Receptors of A.viscosus

采用 3H-标记的粘性放线菌不同菌株 ,对 7种唾液蛋白包被羟基磷灰石的粘附实验 ,来筛选菌毛 及菌毛 的唾液蛋白受体。结果发现 :富脯糖蛋白 (PRPG)、酸性富脯蛋白及富酪蛋白是粘性放线菌菌毛 的受体 ;PRPG、SIg A是菌毛 的受体 ;PRPG是两种菌毛共同的受体 ;同时还发现不同唾液蛋白成份对不同的菌株粘附影响有差异 ,新分离的粘性放线菌菌株的粘附能力比参考株强。

In order to determine the receptors of A.viscosus for adhesion, 7 kinds of main purified proteins from pellicle formed by whole saliva were tested. The result indicated that: ①APRP, statherin, PRPG and SIgA were the receptors of standard and fresh isolated A.viscosus; ②APRP and stather promoted the adherence of 5519 (1\++2\+-) to HA, and SIgA promoted the adherence 5951 (1\+-2\++)to HA; PRPG was the common receptor of the two fimbriae. It suggested that there were receptors not only type 1 fimbriae but also type 2 in pellicle. Two types of fimbriae were involved in the adherence of A.viscosus to pellicle. The difference existed in the receptor of adherence and the activity site of the same receptor.\;