经胰蛋白酶消化真皮皮浆培养成纤维细胞

Fibrocyte Culture throngh Trypsin's Digestion of Dermis Pulp

采用手术切除的包皮或其它部位的正常断层皮，经胰蛋白酶消化揭去表皮，真皮成份在适量的细胞培养液中剪成皮浆，将此皮浆混悬液按点状法接种于培养瓶（皿）中，４～６ｈ可贴壁，然后再加入含胎牛血清的细胞培养液继续培养，结果２４ｈ便可观察到新细胞萌出，２周左右细胞增长成片，结果表明：与常用的胶原酶消化法和普通的组织贴块法相比，具有培养周期短等优点．

Adopted were the foreskin cut during the operation and normal split-thick skin from other parts of the body.Epidermis and dermis were separated by the incubation of trypsin.In appropiate cell culture fluid,dermis was minced into pulp,whose suspension was dibbled in culture flasks and nestled closely to the flask wall after 4～6 hour Then,fetal bovine serum was put in to go on culturing.In the result,new fibrocytes were observed in 24 hours and cells became confluent in about 2 weeks.The results showed that the method holds many advantages in comparison with the routine collagenase-digesting method and tissue-sticking method.