抗磷脂抗体作用的抗原组成成分分析研究

THE ANALYSIS AND STUDY OF THE ANTIGEN COMPOSION OF ACL

通过免疫印迹（ＩＢＴ）分析β２糖蛋白Ⅰ（β２ＧＰＩ）与抗磷脂抗体（ＡＣＬ）之间的关系，用抗β２ＧＰＩ抗体与硝酸纤维膜上的β２ＧＰＩ的反应呈现显色沉淀线，但与ＡＣＬ阳性血清反应沉淀线，结果表明ＡＣＬ作用的主要抗原成份不是β２ＧＰＩ。在修正的ＥＬＩＳＡ法中，稀释液和封闭液中不含牛血清无（β２ＧＰＩ），用ＣＬ包板、与ＡＣＬ的反应降低，在加入β２ＧＰＩ后，反应明显增加，结果表明ＡＣＬ作用的抗原成份是β２ＧＰＩ与ＣＬ形成的复合抗原，用修正的ＥＬＩＳＡ法检测２２例ＡＣＬ阳性血清，其组内变异为１０．９３％，组间变异为１６．７％，重复性８９％。

The relation between β 2GPI with anticardiolipin antibodies (ACL) and cardiolipin (CL) was analysed by immune bloting.The result showed that the target antigen of ACL was not β 2GPI.The purified ACL IgG from APS patients showed little binding in the absence of β 2GPI,OD( x±s )0 022±0 009.As the β 2GPI was added,which enhanced signicantly binding of ACL to CL,OD( x±s )0 218±0 08.There was difference markly by statistical analysis, P <0 01.The result indicated that ACL had recognized likely β 2GPI CL complex. The modified ELISA was studied.Intrassay and interassay coffcients of variation was 10 9%,16 7%.