清开灵不同组分对大鼠MCAO模型脑组织中肿瘤坏死因子的影响特征

Effects of Different Compositions of Qing Kai Ling Injection on Tumor Necrosis Factor in Brain of Rats with MCAO

目的:观察清开灵不同组分对MCAO大鼠脑组织肿瘤坏死因子-α(TNF-α)及可溶性肿瘤坏死因子受体(sTNF-R)含量的影响,探讨其抗脑缺血损伤的机制。方法:采用MCAO模型,应用RIA检测脑组织匀浆肿瘤坏死因子-α(TNF-α含量,ELSIA检测脑组织sTNF-R含量。结果:缺血24h,胆酸组、栀子苷组、珍珠母组及合方组的脑组织中TNF-α含量显著降低(与模型组比P<0.05或P<0.01)。缺血12h,珍珠母组、黄芩苷组和胆酸组脑组织中sTNF-R的含量显著高于模型组(P<0.05或P<0.01);而缺血24h,胆酸组与合方组脑组织中sTNF-R的含量显著低于模型组(P<0.05或P<0.01)。结论:清开灵四种组分及合方可以抑制TNF-α的合成与分泌,对脑缺血后的级联反应具有阻抑作用;而各不同组分对sTNF-R的作用较为复杂,在缺血12h,珍珠母、黄芩苷和胆酸可刺激sTNF-R的合成与分泌,而到缺血后24h,各给药组脑组织中sTNF-R的含量却表现为下降趋势。

Objective： To study the effect of the different ingredients of Qing Kai Ling Injection on the contents of TNF-α and sTNF-R in the brain of rats with MCAO,and to investigate the mec ＇hanism of the different ingredients of Qing Kai Ling Injection in counteracting the damage induced by cerebral isehemic. Methods： The rats were randomly divided into two groups （ 12h and 24h of isehemia）, and then divided into seven subgroups： sham-operated group, ischemic group, cholalic acid-treatment group, baicalin-treatment group, concha margaritifera usta-treatment group, jasminoidin-treatment group and the compatibility of the four ingredients -treatment group. Focal cerebral ischemia models were established by permanent occlusion of left middle cerebral artery （PMCAO） in rats. Radioimmunoassay（RIA）was used to investigate the content of tumor necrosis factor-alpha（TNF-α）in brain tissue of rats with cerebral isehemia. Enzyme-linked immunosorbent assay （ELISA） was used to detect the content of soluble tumor necrosis factor-alpha receptor. Results： After 24h of isehemia, the content of TNF-α of cholalic acid-treatment group, concha margaritifera usta-treatment group, jasminoidin-treatment group and the compatibility of the four ingredients-treatment group decreased remarkably. After 12h of isehemia, compared with model group, the content of soluble tumor necrosis factor-alpha receptor of concha morrgaritifera usta-treatment group, baicalin-treatment group and Cholalic acid-treatment group increased apparently. After 24h of ischemia, compared with model group, the content of sTNF-R in both cholalic acid-treatment group and compatible group decreased apparently. Conclusion： The four kinds of different compositions of Qing Kai Ling Injection on and the compatibility of the four ingredients can repress the synthesis and secretion of TNF-α and can inhibit the cascade reaction after cerebral isehemia. However, the effect of the different ingredients of Qing Kai Ling Injection on the content of sTNF-R is complicated. After 12h of ischemia, cholalic acid, baicalin and concha margaritifera can promote the the synthesis and secretion of sTNF-R, while the content of sTNF-R decreased in all treated group after 24h of ischemia.