补肾固表方对复感儿Th细胞培养上清液中γ-干扰素与白细胞介素-4蛋白含量的影响

Influence of Bushengubiao Prescription on IFN-γ and IL-4 Protein Content in Culture-supernatant of Th Cells in Patients with RRTI

目的:观察补肾固表方对反复呼吸道感染间歇期患儿外周血Th1、Th2特征性细胞因子的影响。方法:采集20例复感儿治疗前后外周血,采用流式细胞仪分选CD4+T细胞,培养36h后,ABC-ELISA法检测其培养上清液中的γ-干扰素(IFN-γ)、白细胞介素-4(IL-4)蛋白含量,并与10例健康对照组进行比较。结果:补肾固表方治疗后IFN-γ升高(P<0.01),IL-4下降(P<0.01),与正常组相比,无显著性差异(P>0.05)。结论:补肾固表方可使患儿IFN-γ含量增加,IL-4含量下降,使Th细胞因子的蛋白表达恢复正常。

Objective： To observe the influence of Bushengubiao Prescription on Th1 and Th2-typed cytokines in children of Recurrent respiratory tract infections （RRTI） in remission stage. Methods： CD4＋ T cells of peripheral blood in 20 patients of RRTI were selected before and after treatment by flow eytometer, then the IFN-γ, 1L-4 protein contents in the culture-supematant were test by ABC-ELISA after 36hr culture, and compared with 10 healthy controls. Results： Level of IFN-γ increased after treatment （ P 〈 0.01 ）, IL-4 decreased （ P 〈 0.01 ）. No significant difference with healthy children （ P 〉 0.05）. Conclusion： Bushengubiao Prescription can get IFN-γ increased and IL-4 decreased then regains the balance of Th eytokines protein.