“肺气虚”大鼠模型肺、皮肤、大肠Fas、Fas-L表达相关性的实验研究

Experimental Study on Expression of Fas and Fas-L of Lung,Skin and Intestine in Deficiency of Lung Qi Model Mice

目的:在分子水平上探讨肺与皮肤、大肠之间的内在联系。方法:单纯烟熏法建立大鼠“肺气虚”病理模型,将20只大鼠随机分为正常对照组和病理模型组。采用免疫组化SP方法,分别检测Fas、Fsa-L在两组动物肺、皮肤和大肠组织中的表达水平,并进行比较。结果:“肺气虚”病理模型组的肺、皮肤、大肠组织中Fas、Fas-L与正常对照组比较明显增高,具有显著性差异(P<0.01)。结论:肺、皮肤、大肠三种组织中Fas、Fas-L的表达水平呈平行相关性,从分子水平上提示肺与皮肤、大肠之间可能存在着内在联系,证实了中医学“肺外合皮毛”及“肺与大肠相表里”的重要理论。

Objective： To study the inner relationship among lung, skin and large intestine in molecule level. Methods： Deficiency of Lung Qi mice models were established by smoking. 20 rats were divided into normal control group and pathologic model group at random. Immunohistochemical method was used to detect the expression of Fas, Fas-L of lung, skins and intestine in two groups respectively. Results： Compared with normal control group, the expression of Fas, Fas - L of lung, skin and intestine of the pathologic model group increased obviously and had significant difference （P 〈 0.01）. Conclusion： The expression levels of Fas, Fas-L in lung, skin and intestine have parallel relativity. It hints that there is close internal contact among lung, skin and intestine in molecule level. Then it confirms the rule of ＂lung governing skin and hair＂ and ＂lung being connected with large intestine＂ on the whole.