杭州石荠苧总黄酮的提取工艺研究

Study on Extraction Method of Total Flavonoids in Hangzhou Population of Mosla hangchouensis Matsuda

目的:优选杭州石荠苧总黄酮提取工艺。方法:采用正交试验设计,以总黄酮含量为指标,考察乙醇浓度、溶媒倍数、提取时间等因素对总黄酮提取率的影响。结果:杭州石荠苧总黄酮的最佳提取工艺为A1B2C2,即加20倍量65%乙醇,回流提取2次,每次1 h。结论:本提取工艺简便可行。

Objective： To study the extraction method of total flavonoids in Mosla hangzhowensis Matsuda. Methods ： Orthogonal experiment design was used, with the content of the total flavonoids as the index, to study the effect of ethanol concentration, solvent quantity and extraction time on recovery rate of total flavonoids. Results： The best extraction condition was： extract two times; adding 20 fold 65 % ethanol and to extract an hour each time. Conclusion： This extraction method is convenient and feasible.