MAGE-12的新B细胞表位抗原性研究

Synthesis and Identification of A new B-cell Epitope of Tumor Antigen MAGE-12

目的:在已设计合成肿瘤抗原MAGE-12的B细胞表位基础上对其进行鉴定。方法:在预测MAGE-12的B细胞表位序列为LEQRSQHCKPEE(3～14)的基础上,采用4分枝多重抗原肽结构设计合成抗原肽,免疫C-57BL/6小鼠,产生多克隆抗体,应用ELISA实验检测多克隆抗体是否为人的抗原肽的特异性抗体。结果:免疫C-57BL/6小鼠后可产生MAGE-12的抗原特异性的多克隆抗体,抗体效价最高达1:256,证明为MAGE-12抗原肽的特异性抗体。结论:证明所预测的表位LEQRSQHCKPEE(3～14)为MAGE-12的B细胞新表位,具有抗原特异性。

Objective： To synthesis and identify B-cell epitope of tumor antigen MAGE- 12, Methods： Based on epitope 3 - 14, a mutiple antigenic peptide system （MAP） was synthesized as immunogen and C-57BL/6 mice were immunised with the immunogen and the poly- clonal anti-serum was produced. The polyclonal antibody was identified by using an ELISA assay. Results： The antibody titer was reached to l： 256. The polyclonal antibedy was identified as specific antibedy of MAGE-12. Conclusion： Thoseresuhsshowedthatonepitope3- 14 may be a new B cell epitope of MAGE-12 and it is specific.