IgM类抗HCV独特型抗体(抗HCV-Ab2)的ELISA法检测及意义

DETECTION AND SIGNIFICANCE OF IgM ANTIIDIOTYPE ANTIBODIES AGAINST ANTI HCV(IgM ANTI HCV Ab2)BY ELISA

用抗人μ链单克隆抗体建立的ＥＬＩＳＡ法检测１５２例肝病患者ＩｇＭ类抗ＨＣＶ独特型抗体，甲肝阳性率０％（０／１５），乙肝８．５％（４／４７），丙肝３２．１％（２６／８１），丙肝合并乙肝者２２．２％（２／９）。丙肝组内频率为急肝０％（０／１７），慢性迁延性肝炎３８．１％（８／２１），慢性活动性肝炎４３．８％（１４／３２），肝炎肝硬化３６．４％（４／１１）。本法重复性好，批内变异系数４．６％，批间变异系数８．５％。用纯化人抗ＨＣＶ抗体进行阻断，阻断率＞８５％，丙肝组与乙肝组有显著性差异（Ｐ＜０．０１），与各种自身免疫性疾病无交叉，有较好的特异性和精密度，且与血清中ＩｇＭ抗ＨＣＶ关系密切，提示丙型肝炎ＩｇＭ类抗ＨＣＶ－Ａｂ２具有模拟抗原的作用，是慢性丙型肝炎的重要检测指标并与丙肝慢性化有关。

An enzyme linked immunosorbent assay for detecting IgM antiidiotype antibodies against anti HCV in the sera of hepatitis C patients has been established,which was based on McAb anti μ 81 hepatitis C patients serum samples were detected.The results showed that the positive rates of IgM anti HCV Ab 2 in the patients with acute hepatitis C,chronic persisting hepatitis C,chronic active hepatitis C and hepatocirrhosis were 0%(0/17),38.1%(8/21),43.8%(14/32) and 36.4%(4/11) respectively.These antiidiotypes were closely associated with the appearance of IgM anti HCV antibodies in the patients sera.It was suggested that IgM anti HCV Ab 2 was an important laboratory diagnostic index of chronic hepatitis C and associated with its chronicity.