宫颈癌及慢性宫颈炎ras癌基因产物p21和DNA倍体的研究

ras Oncogene p21 Expression and DNA Ploidy in Cervical Carcinoma and Chronic Cervicitis

应用细胞免疫荧光技术和流式细胞术对宫颈癌和慢性宫颈炎组织ras癌基因产物p21蛋白的表达量进行了定量研究，并分析了rasp21的表达量与DNA倍体和细胞增殖指数的关系。结果显示：宫颈癌rasp21的表达量（荧光指数FI）明显高于慢性宫颈炎组织，rasp21表达的荧光指数随组织学分级升高而增高。DNA倍体和rasp21的表达量有密切关系。

The oncogene ras p21 expression in cervical carcinomas and chronic cervicitis was quantitatively studied by flow cytometry and immunofluorescence methods and the corelationships of ras p21 with DNA ploidy and cellular proliferation were analysed. The result showed that ras p21 expression was higher in carcinoma than in chronic cervicitis. The fluorescence index in ras p21 expression increased with the escalation of histological grade. DNA ploidy correlated with the amount of ras p21 expression.