分离方法及卵巢发育状况对猪次级卵泡分离和采集的影响

Effects of Isolation Method and the Development of Ovary on the Isolation and Collection of Porcine Secondary Follicles

试验以直径在200～300μm的次级卵泡为研究对象,研究了不同分离方法和卵巢不同发育状况对猪次级卵泡分离和采集的影响。结果表明,显微分离法每个卵巢可采集的卵泡数极显著高于剪碎过滤法,显微分离法分离每个卵泡所用的时间极显著低于剪碎过滤法;有黄体和无黄体的卵巢对卵泡采集的影响差异不显著,但有黄体的卵巢分离的卵泡数稍多于无黄体的卵巢。

By using secondary follicle of diameter of 200-300 μ m as an object, we studied the effect of isolation method and the development of ovary on the isolation and collection of porcine secondary follicles. The results indicated that the number of secondary follicle isolated by microdissection is highly significantly more than that of culting filtration （24.75 ± 2.99vs2.60 ± 0.35, P〈0.01 ）, and the time every follicle cost by microdissection is highly significantly lower than that of cutting filtration （5.40 ± 0.44vs15.52 ± 1.18,P〈0.01 ）. There is no significance of collection of follicle between ovary with corpus luteum and without corpus luteum when both using microdissection method （26.10 ± 2.02vs24.35 ± 2.32, P〉0.05 ）, but ovary with corpus luteum will isolate little more follicles than ovary without corpus luteum.